Part:BBa_J72031:Design

{Bmll64} in BBb

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Source

Overlap Extension PCR (Wobble Construction)

Wobble construction of Bmll64 {<HSV!}

Wobble mll64/mll65                (70, EcoRI/BamHI
Sub in pBca9523-Bca1144#5           (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-Bmll64         {<HSV!}
----
mll64    Forward wobble for {<HSV!} (Bmll64)
CCATAGAATTCATGAGATCTCAGCCGGAGCTCGCACCAGAAGACC
mll65    Reverse wobble for {<HSV!} (Bmll64)
CGTTAGGATCCttaGCAGTCTTCCGGGTCTTCTGGTGCGAGC